AOAC 966.23 PDF
Matrices, Fish/Cooked Seafood, Meat/Frozen Cooked Meat Products, Nuts and Nut Products/Tree Nut Meats. Approved By, AOAC. Method Number, recommended by AOAC Official Method (1). The ap- propriate dilutions were analyzed by the AOAC pour plate method (AOAC ) and the SimPlate . AOAC Compendium of Methods for the Microbiological Examination of Foods 4th Edition: Chapter 7. FDA Bacteriological Analytical Manual: Chapter 3.
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Following are some observations and comments for Duncan’s Multiple Range Test for coliforms. The mean comparisons for each method and level indicated a significant difference between the MPN and all other methods at the low levels for coliforms, with no significant difference at high and medium levels. Red and blue gassing colonies were counted at 24 h and the sum of the two was recorded as total coliforms.
Statistical analyses were done by Mr. Also, it should be noted that the results for the oysters werein general, excellent for the E. This study was designed in consultation with Dr. The MPN and Petrifilm EC differed significantly in the cheddar cheese, the ground beef and the yogurt at the low levels. Likewise, the Duncan mean comparisons for E. Bontrager of RCR Scientific. The food groups tested were as follows: References 1 Roth, J.
Procedures were followed in accordance with the protocol established in the collaborative study of the medium 2. Therefore, 15 samples of each of the 20 products were tested by each method. Therefore, various media formulations can be easily made according to the standard accepted ingredients for each individual medium.
However, there was no significant difference among all the other methods for both coliforms and E. The mean comparisons for each method and level of coliforms indicated no significant differences among any of the methods except for Petrifilm EC and VRB Redigel and at the low level.
Redigel is an agar plate substitute whose only basic difference to standard agar plate media is the substitution of a low methoxyl pectin for agar as the gelling agent in the media. Forgot username or password? However, comparison of ColiChrome 2 Redigel with the other 2 rapid methods showed The results of samples of 20 foods were collected and statistically analyzed.
The study was submitted to the AOAC for the purpose of receiving permission to do an official collaborative study with the goal of having ColiChrome 2 Redigel being approved as an official method of the AOAC. The decision to base this study on a comparison of ColiChrome 2 Redigel to the standard MPN method was influenced by the fact that other recent collaborative studies have been done and approved on the basis of this format 5,6. The mean comparisons for coliforms with each method and level indicated no significant differences between the MPN and all other methods.
Also, none of the mean comparisons for each method and level differed significantly. This oyster tissue activity apparently interferes with the obvious expression of red coliform CFUs.
ColiChrome 2 Redigel method for total coliforms and E. ColiChrome 2 Redigel coliforms In two of the foods 966.223 cheese and oystersthe MPN results were significantly different than the other three methods. The mean comparisons for each method and level indicated no significant differences among any of the methods.
Petrifilm plates were incubated at 35 C for 48 h and colonies were counted at 24 and 48 h. In the general linear model for E. However, there was no significant difference between the MPN and other methods for coliforms. Following the introduction of the technology by RCR Scientific, the idea was copied by most of the major media makers worldwide since the RCR patent only covered the U. Therefore, total coliform colonies appear as red colonies in the clear medium.
The mean comparisons for each method and level indicated no woac differences among any of the aoqc except for Petrifilm EC with ColiChrome 2 Redigel and VRB Redigel at the low levels for coliforms.
Precollab Study – Micrology Labs
The mean comparisons for each aoa and level indicated no significant differences among any of the methods except for the MPN and Petrifilm EC at the high level of coliforms. The mean comparisons for each method and level indicated no significant differences 966.23 any of the methods except for MPN and Petrifilm EC at the low and high levels for E.
Based upon the results of this study, we believe that any of the three rapid methods may be used effectively to differentiate and confirm coliforms or E. Also, the Duncan mean comparisons for E. Gas production was considered positive.
Micrology Laboratories currently serves the food and dairy industry with its line of Easygel and Coliscan media. It is our experience that if low populations of coliforms occur in oysters so that 1mL of a 1: Spices – Xoac Products – Nutmeat pepper black ground flour peanuts raw thyme rice walnuts.